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The second reaction cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as items (Fig. 9) - circularly polarized luminescence. As drawn in Fig - https://lwccareers.lindsey.edu/profiles/4273853-julie-ann-desa-lorenz. 9, the response might also include electron transfer from H4B to the Fe, IIO2 intermediate
l9 As displayed in Fig. 9, this could be since the H4B extreme acts as an electron sink in a later point in the reaction, so as to permit NOS to, Fig. 9. A original site model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.